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I got the following "Note" : [05/75069e] NOTE: Process ASSEMBLE_ORGANELLES:MITOHIFI_MITOHIFI (hifiasm-raw-default) terminated with an error exit status (1) -- Error is ignored
It seems there is no mitochondrial contig found but drosophila can have NUMTS
.command.log
2024-12-12 09:32:26 [INFO] Welcome to MitoHifi v2. Starting pipeline...
2024-12-12 09:32:26 [INFO] Length of related mitogenome is: 17535 bp
2024-12-12 09:32:26 [INFO] Number of genes on related mitogenome: 37
2024-12-12 09:32:26 [INFO] Running MitoHifi pipeline in contigs mode...
2024-12-12 09:32:26 [INFO] 1. Fixing potentially conflicting FASTA headers
2024-12-12 09:32:30 [INFO] 2. Let's run the blast of the contigs versus the close-related mitogenome
2024-12-12 09:32:30 [INFO] 2.1. Creating BLAST database:
2024-12-12 09:32:30 [INFO] makeblastdb -in OP035243.1.fasta -dbtype nucl
2024-12-12 09:32:34 [INFO] Makeblastdb done.
2024-12-12 09:32:34 [INFO] 2.2. Running blast of contigs against close-related mitogenome:
2024-12-12 09:32:34 [INFO] blastn -query fixed_header_contigs.fasta -db OP035243.1.fasta -num_threads 12 -out contigs.blastn -outfmt 6 std qlen slen
2024-12-12 09:33:32 [INFO] Blast done.
2024-12-12 09:33:32 [INFO] 3. Filtering BLAST output to select target sequences
2024-12-12 09:33:32 [INFO] Filtering thresholds applied:
2024-12-12 09:33:32 [INFO] Minimum query percentage = 50
2024-12-12 09:33:32 [INFO] Minimum query length = 80% subject length
2024-12-12 09:33:32 [INFO] Maximum query length = 5 times subject length
Attention!
'parsed_blast.txt' and 'parsed_blast_all.txt' files are empty.
The pipeline has stopped !! You need to run further scripts to check if you have mito reads pulled to a large NUMT!
It seems to be a MitoHifi problem
update (16/12): the pipeline seems to be stuck so I killed it
Environment (please complete the following information):
OS: nac
The text was updated successfully, but these errors were encountered:
Describe the bug (12/12)
I am running the pipeline on Drosophila histrio from Darwin tree of life :
https://portal.darwintreeoflife.org/data/root/details/Drosophila%20histrio
I got the following "Note" : [05/75069e] NOTE: Process
ASSEMBLE_ORGANELLES:MITOHIFI_MITOHIFI (hifiasm-raw-default)
terminated with an error exit status (1) -- Error is ignoredIt seems there is no mitochondrial contig found but drosophila can have NUMTS
.command.log
It seems to be a MitoHifi problem
update (16/12): the pipeline seems to be stuck so I killed it
Environment (please complete the following information):
The text was updated successfully, but these errors were encountered: