diff --git a/CODEOWNERS b/CODEOWNERS
index 9b7963aa4f75..073e81cd244a 100644
--- a/CODEOWNERS
+++ b/CODEOWNERS
@@ -847,6 +847,7 @@
 /tests/drivers/uart/                      @nrfconnect/ncs-low-level-test
 /tests/drivers/sensor/multicore_temp/     @nrfconnect/ncs-low-level-test
 /tests/drivers/sdp_asm/                   @nrfconnect/ncs-low-level-test @nrfconnect/ncs-ll-ursus
+/tests/drivers/nrf_qspi_flash/            @nrfconnect/ncs-low-level-test
 /tests/lib/at_cmd_parser/                 @nrfconnect/ncs-modem
 /tests/lib/at_cmd_custom/                 @nrfconnect/ncs-modem
 /tests/lib/at_parser/                     @nrfconnect/ncs-modem
diff --git a/scripts/ci/tags.yaml b/scripts/ci/tags.yaml
index d1fc4598ce83..606f45924612 100644
--- a/scripts/ci/tags.yaml
+++ b/scripts/ci/tags.yaml
@@ -1206,6 +1206,7 @@ ci_tests_drivers_flash:
     - nrf/tests/drivers/flash/
     - nrfxlib/nrf_rpc/
     - zephyr/drivers/flash/
+    - nrf/tests/drivers/nrf_qspi_flash/
 
 ci_tests_unity:
   files:
diff --git a/tests/drivers/nrf_qspi_flash/CMakeLists.txt b/tests/drivers/nrf_qspi_flash/CMakeLists.txt
new file mode 100644
index 000000000000..6d779a563528
--- /dev/null
+++ b/tests/drivers/nrf_qspi_flash/CMakeLists.txt
@@ -0,0 +1,13 @@
+#
+# Copyright (c) 2025 Nordic Semiconductor ASA
+#
+# SPDX-License-Identifier: LicenseRef-Nordic-5-Clause
+#
+
+cmake_minimum_required(VERSION 3.20.0)
+
+find_package(Zephyr REQUIRED HINTS $ENV{ZEPHYR_BASE})
+
+project(nrf_qspi_flash)
+
+target_sources(app PRIVATE ${ZEPHYR_BASE}/samples/drivers/spi_flash/src/main.c)
diff --git a/tests/drivers/nrf_qspi_flash/flash_cfg/mx25r64_single_line.overlay b/tests/drivers/nrf_qspi_flash/flash_cfg/mx25r64_single_line.overlay
new file mode 100644
index 000000000000..066f9d0747bc
--- /dev/null
+++ b/tests/drivers/nrf_qspi_flash/flash_cfg/mx25r64_single_line.overlay
@@ -0,0 +1,11 @@
+/*
+ * Copyright (c) 2025 Nordic Semiconductor ASA
+ *
+ * SPDX-License-Identifier: LicenseRef-Nordic-5-Clause
+ */
+
+&mx25r64 {
+	status="okay";
+	readoc="fastread";
+	writeoc="pp";
+};
diff --git a/tests/drivers/nrf_qspi_flash/prj.conf b/tests/drivers/nrf_qspi_flash/prj.conf
new file mode 100644
index 000000000000..f6f9bf317a22
--- /dev/null
+++ b/tests/drivers/nrf_qspi_flash/prj.conf
@@ -0,0 +1,8 @@
+CONFIG_FLASH=y
+CONFIG_ASSERT=y
+
+CONFIG_PRINTK=y
+CONFIG_LOG=y
+CONFIG_CONSOLE=y
+CONFIG_UART_CONSOLE=y
+CONFIG_SERIAL=y
diff --git a/tests/drivers/nrf_qspi_flash/testcase.yaml b/tests/drivers/nrf_qspi_flash/testcase.yaml
new file mode 100644
index 000000000000..a3b8159c16de
--- /dev/null
+++ b/tests/drivers/nrf_qspi_flash/testcase.yaml
@@ -0,0 +1,46 @@
+common:
+  sysbuild: true
+  depends_on: spi
+
+tests:
+  tests.drivers.nrf_qspi_flash.qspi_multi_line_mode:
+    tags:
+      - ci_tests_drivers_flash
+      - drivers
+      - flash
+      - spi
+    platform_allow:
+      - nrf52840dk/nrf52840
+      - nrf5340dk/nrf5340/cpuapp
+    integration_platforms:
+      - nrf52840dk/nrf52840
+      - nrf5340dk/nrf5340/cpuapp
+    harness: console
+    harness_config:
+      type: multi_line
+      ordered: true
+      regex:
+        - ".*Flash erase succeeded.*"
+        - ".*Data read matches data written. Good.*"
+
+  tests.drivers.nrf_qspi_flash.qspi_single_line_mode:
+    tags:
+      - ci_tests_drivers_flash
+      - drivers
+      - flash
+      - spi
+    platform_allow:
+      - nrf52840dk/nrf52840
+      - nrf5340dk/nrf5340/cpuapp
+    integration_platforms:
+      - nrf52840dk/nrf52840
+      - nrf5340dk/nrf5340/cpuapp
+    extra_args:
+      - EXTRA_DTC_OVERLAY_FILE="flash_cfg/mx25r64_single_line.overlay"
+    harness: console
+    harness_config:
+      type: multi_line
+      ordered: true
+      regex:
+        - ".*Flash erase succeeded.*"
+        - ".*Data read matches data written. Good.*"