diff --git a/src/python_testing/TC_CNET_4_10.py b/src/python_testing/TC_CNET_4_10.py
new file mode 100644
index 00000000000000..8abf72f129408b
--- /dev/null
+++ b/src/python_testing/TC_CNET_4_10.py
@@ -0,0 +1,241 @@
+#
+#    Copyright (c) 2025 Project CHIP Authors
+#    All rights reserved.
+#
+#    Licensed under the Apache License, Version 2.0 (the "License");
+#    you may not use this file except in compliance with the License.
+#    You may obtain a copy of the License at
+#
+#        http://www.apache.org/licenses/LICENSE-2.0
+#
+#    Unless required by applicable law or agreed to in writing, software
+#    distributed under the License is distributed on an "AS IS" BASIS,
+#    WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied.
+#    See the License for the specific language governing permissions and
+#    limitations under the License.
+#
+
+import chip.clusters as Clusters
+from chip.testing.matter_testing import MatterBaseTest, TestStep, default_matter_test_main, has_feature, run_if_endpoint_matches, async_test_body
+from mobly import asserts
+
+
+class TC_CNET_4_10(MatterBaseTest):
+    def def_TC_CNET_4_10(self):
+        return '[TC-CNET-4.10] [Thread] Verification for RemoveNetwork Command [DUT-Server]'
+
+    def pics_TC_CNET_4_10(self):
+        return [
+            'CNET.S',
+            'CNET.S.F01'
+        ]
+
+    def steps_TC_CNET_4_10(self):
+        return [
+            TestStep("preconditions", "TH is commissioned", is_commissioning=True),
+            TestStep(1, "TH reads from the DUT the Network Commissioning Cluster FeatureMap to verify Thread support"),
+            TestStep(2, "TH sends ArmFailSafe command to the DUT with ExpiryLengthSeconds set to 900"),
+            TestStep(3, "TH reads Networks attribute from the DUT and save the number of entries as 'NumNetworks'"),
+            TestStep(4, "TH sends RemoveNetwork Command to the DUT with NetworkID field set to PIXIT.CNET.THREAD_1ST_OPERATIONALDATASET and Breadcrumb field set to 1"),
+            TestStep(5, "TH reads Networks attribute from the DUT"),
+            TestStep(6, "TH reads LastNetworkingStatus attribute from the DUT"),
+            TestStep(7, "TH reads LastNetworkID attribute from the DUT"),
+            TestStep(8, "TH reads Breadcrumb attribute from the General Commissioning cluster"),
+            TestStep(9, "TH sends ConnectNetwork command to the DUT with NetworkID field set to PIXIT.CNET.THREAD_1ST_OPERATIONALDATASET"),
+            TestStep(10, "TH reads Breadcrumb attribute from the General Commissioning cluster"),
+            TestStep(11, "TH sends ArmFailSafe command to the DUT with ExpiryLengthSeconds set to 0"),
+            TestStep(12, "TH reads Networks attribute from the DUT"),
+            TestStep(13, "TH sends ArmFailSafe command to the DUT with ExpiryLengthSeconds set to 900"),
+            TestStep(14, "TH sends RemoveNetwork Command to the DUT"),
+            TestStep(15, "TH sends CommissioningComplete command to the DUT"),
+            TestStep(16, "TH sends ArmFailSafe command to the DUT with ExpiryLengthSeconds set to 0"),
+            TestStep(17, "TH reads Networks attribute from the DUT")
+        ]
+
+    @async_test_body
+    async def test_TC_CNET_4_10(self):
+        # Commissioning is already done
+        self.step("preconditions")
+        
+        cnet = Clusters.NetworkCommissioning
+        gen_comm = Clusters.GeneralCommissioning
+        thread_network_id = "hex:1111111122222222"  # From PIXIT.CNET.THREAD_1ST_OPERATIONALDATASET
+
+        # Step 1: Verify FeatureMap indicates Thread support
+        self.step(1)
+        feature_map = await self.read_single_attribute_check_success(
+            cluster=cnet,
+            attribute=cnet.Attributes.FeatureMap
+        )
+        asserts.assert_equal(feature_map, 2, "FeatureMap must be 2 for Thread interface")
+
+        # Step 2: Arm failsafe and verify response
+        self.step(2)
+        response = await self.send_single_cmd(
+            cluster=gen_comm,
+            cmd=gen_comm.Commands.ArmFailSafe(expiryLengthSeconds=900, breadcrumb=0)
+        )
+        asserts.assert_equal(response.ErrorCode, 0, "ArmFailSafe command failed")
+
+        # Step 3: Read Networks and verify thread network
+        self.step(3)
+        networks = await self.read_single_attribute_check_success(
+            cluster=cnet,
+            attribute=cnet.Attributes.Networks
+        )
+        num_networks = len(networks)
+
+        # Find network index
+        userth_netidx = None
+        for idx, network in enumerate(networks):
+            if network.NetworkID == thread_network_id:
+                userth_netidx = idx
+                asserts.assert_true(network.Connected, "Thread network not connected")
+                break
+        asserts.assert_true(userth_netidx is not None, "Thread network not found")
+
+        # Step 4: Remove network
+        self.step(4)
+        response = await self.send_single_cmd(
+            cluster=cnet,
+            cmd=cnet.Commands.RemoveNetwork(
+                networkID=thread_network_id,
+                breadcrumb=1
+            )
+        )
+        asserts.assert_equal(response.NetworkingStatus,
+                             cnet.Enums.NetworkCommissioningStatusEnum.kSuccess, "Network was not removed")
+        asserts.assert_equal(response.NetworkIndex, userth_netidx,
+                             "Incorrect network index in response")
+
+        # Step 5: Verify network count reduced
+        self.step(5)
+        networks = await self.read_single_attribute_check_success(
+            cluster=cnet,
+            attribute=cnet.Attributes.Networks
+        )
+        asserts.assert_equal(len(networks), num_networks - 1,
+                             "Network count not reduced")
+
+        # Step 6: Check LastNetworkingStatus
+        self.step(6)
+        status = await self.read_single_attribute_check_success(
+            cluster=cnet,
+            attribute=cnet.Attributes.LastNetworkingStatus
+        )
+        expected_status = cnet.Enums.NetworkCommissioningStatusEnum.kSuccess
+        asserts.assert_equal(status, expected_status)
+
+        # Step 7: Check LastNetworkID
+        self.step(7)
+        last_network_id = await self.read_single_attribute_check_success(
+            cluster=cnet,
+            attribute=cnet.Attributes.LastNetworkID
+        )
+        expected_network_id = thread_network_id
+        asserts.assert_equal(last_network_id, expected_network_id)
+
+        # Step 8: Verify breadcrumb
+        self.step(8)
+        breadcrumb = await self.read_single_attribute_check_success(
+            cluster=gen_comm,
+            attribute=gen_comm.Attributes.Breadcrumb
+        )
+        asserts.assert_equal(breadcrumb, 1)
+
+        # Step 9: Try to connect to removed network
+        self.step(9)
+        response = await self.send_single_cmd(
+            cluster=cnet,
+            cmd=cnet.Commands.ConnectNetwork(
+                networkID=thread_network_id,
+                breadcrumb=2
+            )
+        )
+        asserts.assert_equal(response.NetworkingStatus,
+                             cnet.Enums.NetworkCommissioningStatusEnum.kNetworkIDNotFound)
+
+        # Step 10: Verify breadcrumb unchanged
+        self.step(10)
+        breadcrumb = await self.read_single_attribute_check_success(
+            cluster=gen_comm,
+            attribute=gen_comm.Attributes.Breadcrumb
+        )
+        asserts.assert_equal(breadcrumb, 1)
+
+        # Step 11: Disable failsafe
+        self.step(11)
+        response = await self.send_single_cmd(
+            cluster=gen_comm,
+            cmd=gen_comm.Commands.ArmFailSafe(expiryLengthSeconds=0, breadcrumb=0)
+        )
+        asserts.assert_equal(response.ErrorCode, 0)
+
+        # Step 12: Verify network restored
+        self.step(12)
+        networks = await self.read_single_attribute_check_success(
+            cluster=cnet,
+            attribute=cnet.Attributes.Networks
+        )
+        asserts.assert_equal(len(networks), num_networks,
+                             "Network count not restored")
+        found = False
+        for network in networks:
+            if (network.NetworkID == thread_network_id and
+                    network.Connected):
+                found = True
+                break
+        asserts.assert_true(found, "Thread network not restored")
+
+        # Step 13: Re-arm failsafe
+        self.step(13)
+        response = await self.send_single_cmd(
+            cluster=gen_comm,
+            cmd=gen_comm.Commands.ArmFailSafe(expiryLengthSeconds=900, breadcrumb=0)
+        )
+        asserts.assert_equal(response.ErrorCode, 0)
+
+        # Step 14: Remove network again
+        self.step(14)
+        response = await self.send_single_cmd(
+            cluster=cnet,
+            cmd=cnet.Commands.RemoveNetwork(
+                networkID=thread_network_id,
+                breadcrumb=1
+            )
+        )
+        asserts.assert_equal(response.NetworkingStatus,
+                             cnet.Enums.NetworkCommissioningStatusEnum.kSuccess)
+        asserts.assert_equal(response.NetworkIndex, userth_netidx)
+
+        # Step 15: Complete commissioning
+        self.step(15)
+        response = await self.send_single_cmd(
+            cluster=gen_comm,
+            cmd=gen_comm.Commands.CommissioningComplete()
+        )
+        asserts.assert_equal(response.ErrorCode, 0)
+
+        # Step 16: Verify failsafe disabled
+        self.step(16)
+        response = await self.send_single_cmd(
+            cluster=gen_comm,
+            cmd=gen_comm.Commands.ArmFailSafe(expiryLengthSeconds=0, breadcrumb=0)
+        )
+        asserts.assert_equal(response.ErrorCode, 0)
+
+        # Step 17: Verify network remains removed
+        self.step(17)
+        networks = await self.read_single_attribute_check_success(
+            cluster=cnet,
+            attribute=cnet.Attributes.Networks
+        )
+        asserts.assert_equal(len(networks), num_networks - 1,
+                             "Incorrect network count")
+        for network in networks:
+            asserts.assert_not_equal(network.NetworkID, thread_network_id,
+                                     "Network still present after removal")
+
+
+if __name__ == "__main__":
+    default_matter_test_main()
diff --git a/src/python_testing/test_metadata.yaml b/src/python_testing/test_metadata.yaml
index 5bd91ecf542152..25b3878bbedbfd 100644
--- a/src/python_testing/test_metadata.yaml
+++ b/src/python_testing/test_metadata.yaml
@@ -5,6 +5,8 @@ not_automated:
       reason: Code/Test not being used or not shared code for any other tests
     - name: TC_CNET_4_4.py
       reason: It has no CI execution block, is not executed in CI
+    - name: TC_CNET_4_10.py
+      reason: It has no CI execution block, is not executed in CI
     - name: TC_DGGEN_3_2.py
       reason:
           src/python_testing/test_testing/test_TC_DGGEN_3_2.py is the Unit test