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RQCee: Evaluation of Response Curves from Preprocessed MS Data

Welcome to RQCee, a Shiny application for evaluating response curves from preprocessed mass spectrometry (MS) data. This tool enables users to upload data, visualize plots, and download analysis results.

Online Version of the App

The online version of this app can be access via https://slinghub.shinyapps.io/RQCee

Features

  • Data Upload: Accepts CSV and TSV files from MRMkit and MH Quant.
  • Plot Layout: Customizable with user-defined rows and columns.
  • Data Annotation: Interactive tables for data selection and filtering.
  • Visualization: Generate and download detailed plots of response curves.
  • Statistics: Access and export statistical analyses in Excel format.

Dependencies

The application requires the following R packages:

  • shiny
  • bslib
  • shinyjs
  • rhandsontable
  • DT
  • ggplot2
  • midar
  • tidyverse
  • dplyr
  • stringr
  • shinyWidgets
  • openxlsx2

How to Use

  1. Select Format: Choose between MRMkit or MH Quant.
  2. Upload Data: Upload your MS data files via the sidebar.
  3. Customize Plot Layout: Set the number of rows and columns for plot arrangement.
  4. Annotate Data: Use the annotations tab to apply or clear selections.
  5. Retrieve Plots & Statistics: Navigate the plots and statistics tabs to generate and view results.
  6. Download Results: Export plots as PDFs and statistics as Excel files.

Additional Information

  • RQCee offers a user-friendly interface for comprehensive MS data analysis, ensuring intuitive data processing and result accessibility.
  • For further assistance or inquiries, please contact the development team: Shanshan Ji (lsijish@nus.edu.sg) or Bo Burla (bo.burla@nus.edu.sg).

Ensure your data is formatted correctly to fully utilize RQCee's capabilities. Happy analyzing!

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