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This PR adds a third FASTQ file which contains UMI sequences, which matches the read structure of some library preparation kits which embed the UMI in an index sequence.

It adds the third UMI to the samplesheet for testing when running fastquorum in -profile test mode. Question for @nh13, shall we create a distinct samplesheet for running this use case? When combined with nf-test we can test a range of inputs.

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famosab commented Mar 11, 2025

@adamrtalbot Bump :) Do you still need this?

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2 participants